Kinetics study of hydrochlorothiazide lactose liquid state interaction using conventional isothermal arrhenius method under basic and neutral conditions

The Maillard reaction of hydrochlorothiazide (HCTZ) and lactose has been previously demonstrated
in pharmaceutical formulations. In this study, the activation energy of – hydrohlorothiazide and lactose
interaction in the liquid state was ascertained under basic and neutral conditions. Conventional isothermal
High Performance Liquid Chromatography (HPLC) technique was employed to ascertain the kinetic
parameters using Arrhenius method. Results: The activation energy obtained was 82.43 and 100.28
kJ/mol under basic and neutral conditions, respectively. Consequently, it can be inferred that Maillard
reaction is significantly affected by pH, which can be used as a control factor whenever the reaction
potentially occurs.
Uniterms: Hydrochlorothiazide/lactose/Maillard reaction. Hydrochlorothiazide/lactose/interaction/
kinetic parameters. High Performance Liquid Chromatography (HPLC)/kinetic parameters study.