The prevalence of methicillin-resistant Staph. Aureus strains producing enterotoxin A and B

Staphylococcus aureus is a gram positive coccus which is able to cause
different kinds of infection in certain condition. The function of this bacteria is to provide
the conditions for the invasion of it to the host with the secretion of different sorts
of toxins such as Staphylococcus aureus enterotoxin, including important virulence factors
that super antigens are all factors digestive inconvenience. Staphylococcus aureus
enterotoxin-secreting toxins such conditions provides invasion of host genes. There are
different types of SE, but type A enterotoxin (SEA) and type B enterotoxin (SEB) are
the most important types. Therefore, in this study, the prevalence of Staphylococcus aureus
toxin-producing enterotoxin genes (SEB, SEA) in clinical strains isolated from patients
in teaching hospitals of Shahrekord city, Iran, were studied.
Methods: This cross-sectional and descriptive study, which was conducted from May
2014 to December 2014. A hundred and ten isolates of Staphylococcus aureus from patients
collected over a period of 8 months and were first identified using standard biochemical
methods and laboratory. Using standard methods and laboratory tests were
identified and compared with the antibiotic oxacillin minimum inhibitory concentration
were determined by broth micro dilution, and then they were assessed by polymerase
chain reaction (PCR) technique.
Results: The results indicated that, 110 samples of dairy products infected by Staphylococcus
aureus were detected. Two cases (1.8%) of these infected samples were carrying
both enterotoxin A and enterotoxin B genes. The frequencies of enterotoxin A genes
were twenty-six cases (23/6%) and The frequencies of enterotoxin B genes were two
cases (1/8%), respectively.
Conclusion: The detection of enterotoxin A and enterotoxin B genes, shows the most
important role they have in bringing about superinfection. The detection of enterotoxin
A and B genes, shows the most important role they have in bringing about superinfection.
Enterotoxins SEA and SEB are heat stable; therefore heating has no effect on
dairy products contaminated by enterotoxins and gastritis may occur in a short period of
time. As PCR is a rapid, sensitive, specific and inexpensive method, we suggest that it
can be replaced to traditionally assays for detecting Staphylococcus aureus enterotoxin.