STUDY ON THE PREVALENCE OF CTX-M9 GENE IN STRAINS OF ESCHERICHIA COLI TO BETA-LACTAM ANTIBIOTICS RESISTANT IN SANANDAJ CITY

β-lactamase enzymes, the most important factor to antibiotic resistance among
Gram-negative bacteria, is of beta-lactam family. Today, we experience increasing infection in the world
and this is one of the emerging health problems in the world. CTX-M9 is a beta-lactamase resistance
gene. The aim of this study was to determine the prevalence of ESBL producing Escherichia and
molecular evaluation of CTX-M9 β-lactamase was amplified by PCR.
Materials & Methods: In a series 270 urine samples were collected from medical centers in the city of
Sanandaj, the EMB-agar medium at 37 ° C for 24 hours and biochemical tests to confirm the 270
samples, 100 isolates of Escherichia coli was isolated. Antibiotic susceptibility using disk diffusion and
ESBL enzymes were determined using the combined disk. The presence of CTX-M9 using specific
primers was detected by PCR method.
Results: The drug-resistance of isolates to11 antibiotics was obtained. 27 (27%) ESBLs producing
strains were detected by using combined disc method on 100 strains. PCR used for the detection of
CTX-M-9 gene showed that 10 (34%) out of 27 isolates contained such gene.
Conclusion: The rate of ESBLs producing strains in the present study and various reports from other
countries is highly increasing, therefore, using an appropriate treatment protocol based on the
antibiogram pattern of the strains is highly recommended. The results of PCR showed a high percentage
of β-lactamase resistant E. coli strains. So identification of ctx-M9 genes and Prevalence of this Genes
can accelerate diagnosing and treatment of patients