EVALUATION OF ANTI-PROLIFERATIVE AND APOPTOTIC EFFECT OF RESVERATROL ON AGS CELL LINE OF GASTRIC ADENOCARCINOMA

Resveratrol (3,4,5 tri-hydroxy-trans-acetylben-1acetyl benoid) is a phenolic
natural product which is found in red grapes also in Japanese knotweed root (polygonum cuspidatum).
Resveratrol is also found in Gnetum cleistostachyum. In recent years, many researches were about antiinflammatory
and anti carcinogenic effect of resveratrol especially in breast, colon and esophageal
cancer, but a few researches were about its role in gastric adenocarcinoma. This research has been done
about the role of resveratrol in an in vitro model.
Materials & Methods: For proliferation study, we used MTT assay procedure and for apoptosis, anexinpropidium
iodide flowcytometery method has been done. Also we have used AGS cell line cultured in
RPMI medium in 5% CO2 370C in 96 wells culture plate, the cells incubated with resveratrol and
cisplatin (as positive control) for 24 and 48 hr. in different concentrations, IC50 has been determined
and compared with control.
Results: Our results showed that reveratrol in IC50 concentration( 40.6 μM) has significant
antiprolifrative effect in AGS cells compared with control which was in parallel with cisplatin. Also at
IC50 concentration 6.75% apoptosis and 10.8% necrosis has been observed after 24 hr. incubation
Conclusion: According to our results resveratrol has significant antiproliferative and apoptotic effect as
in vitro model of gastric adenocarcinoma and can be suggested for co-treatment in future chemotherapy
of gastric malignancies