THE EFFECT OF NICKEL ON THE STRUCTURE OF KINASE REGION OF TYPE 2 FIBROBLAST GROWTH FACTOR RECEPTOR

Fibroblast growth factor receptor type II (FGFR2b) is the essential factor of
cellular signal transduction that regulates important biological processes including cell proliferation
and differentiation. The inpairment in the signaling of these receptors is associated with several human
pathology. Various factors including toxic metals can change the signaling pathways. This study was
aimed to investigate the effects of Nickel as the toxic metal on the structure of FGFR2b.
Materials & Methods: In this study, by using plasmid pLEICS-01, BL21 bacterial, induction of IPTG,
electrophoresis and column containing Ni2+ -NTA the recombinant FGFR2b was expressed and
purified. The effects of Ni concentrations under 100 mM on the structure of the protein kinase domain
by Spectrofluorometer and Circular dichroism (CD) was studied.
Resultas: The nickel concentrations (under 100 mM) do not have a significant impact on the regular
secondary structure of the protein. But they show a minor change in natural emission of tryptophan in
the tertiary structure.
Conclusion: The main structure and stability kinase domain of the fibroblast growth factor receptor
type II does not change by nickel.