DIRECT SANDWICH ELISA METHOD FOR DIAGNOSIS OF STOMACH ADENOCARCINOMA

Solid tumors in their course of development have the stages of dysplasia,
carcinoma insitu, angiogenesis, invasion and metastasis. In this process, peptide components having
the ability of angiogenesis inhibition (angiostatin) are produced and due to their low molecular weight,
they are filtrated. The purpose of this study is to study the probability of primary diagnosis of stomach
adenocarcinoma in patients by the application of the filtered peptides in urine to introduce a noninvasive and safe method.
Materials & Methods: The randomized urine samples of 20 patients with stomach adenocarcinoma
were compared with normal urine samples of 20 healthy people by means of the optimized direct
sandwich ELISA in order to diagnose the peptide components.
Results: The results by means of T-test (P value< 0.05) showed a significant correlation between
stomach adenocarcinoma and finding of these peptides in urine and they were completely in
accordance with the results of pathologic tests.
Conclusion: The comparison between results obtained in this study showed that by finding the
peptides in patients' urine, a non-invasive and safe method was used for primary detection of stomach
adenocarcinoma in suspicious patients which was performable before invasive methods (pathology);
nevertheless, it is needed to confirm it with complementary tests similar to each screening method.