HISTOMORPHOMETRIC AND IMMUNOHISTOCHEMICAL EVALUATION OF CELL TRANSPLANTATION ON THE REPAIR OF BONE DEFECTS IN RAT

Bone marrow stromal cells (BMSCs) are multipotent stem cells that can
proliferate and differentiate into osteogenic cells. This study aimed to investigate the effects of
BMSCs transplantation for the repair of bone defects in rat.
Materials & Methods: In this study twenty-eight male adult rats were equally divided into 4 groups.
Group 1 (control group) received no treatment after bone defect; after bone defect the culture medium
was injected at the site of bone defect for group 2 (sham group); group 3 was non-autologous cell
transplantation group and group 4 was autologous cell transplantation group. Bone histomorphometric
evaluations and cell counting were performed in all groups.
Results: No significant differences were found between the control group and Sham according to
histomorphometric study. The mean trabecular surface was significantly increased in autologous and
non-autologous groups compared to the control group. In addition, mean number of osteocytes was
increased in autologous and non-autologous groups when compared to the control group.
Conclusion: It seems that both autologous and non-autologous BMSCs transplantation appears to be
effective in the repair of bone defect.