ANTIBIOGRAM AND GENOTYPE OF PSEUDOMONAS AEROGINOSA IN CYSTIC FIBROSIS

Cystic Fibrosis is one of the most prevalent fatal autosomal recessive diseases
among white children. Mutation in CFTR gene cause ion imbalance of membranes and reduce the
fluid in the face of main pulmonary airways which in turn provide proper medium for opportunistic
bacteria specially pseudomonasaeroginosa and may lead to lung dysfunction. The aim of this study
was to state antibiogram and genotype of pseudomonasaeroginosa in cystic fibrosis patients.
Material & Methods: In this cross sectional study, we obtained specimen of sputum or deep
pharyngeal swaps from 46 patients for determining the bacterial species. We used standard lineage P.
aeruginosa ATCC27853 for antibiotic susceptibility test and one type of B. cepacia for standardization
and comparison. We also used 11 antibiotic discs for antibiogram. The method for determination of
genetic lineage was RAPD-PCR.
Results: from the total of 46patients with fibrous cystic 58. 7% were male and 48. 3% were female.
There were 20 patients (74. 1%) in the male group and 11 patients (57. 9%) in the female group with
pseudomonasaeroginosa colonization and there was not B. cepacia colonization. There were 9 patients
with staphylococcus aureous, 7 with Klebsiela, 11 with Candida albicans, and 1 with Serachia
marsensis colonization. The pseudomonas aeroginosa was detected in 31 patients (67. 4%) which had
three lineages: mucoid (15 patients), non-mucoid (12 patients), and mucoid & non-mucoid (4
patients). Antibiogram shows the sensitivity to Tobramycin and Ciprofloxacin in all patients; there
was also sensitivity to Amikacin (7. 8%), Piperacilin (93. 3%), Gentamicin (91. 1%), Ticarcilin (86.
75), Colicitin (80%), Carbenicilin (48. 9%), Cefotaxim (26. 7%), Imipenem (26. 7%), and Ceftazidim
(11. 1%). There was hemogenocity in genetic phenotyping with RAPD-PCR method.
Conclusion: This study showed high resistant to Imipenem and Cephalosporins in
pseudomonaslineages. There was not any relation between genotype and antibiogram and
demographic characteristics of patients (sex and age). In addition, there was no difference among
species according to potency and transmission properties.