BACTERICIDAL EFFECTS OF EUCALYPTUS GLOBULUS EXTRACTS ON INTRAMACROPHAGE BRUCELLA ABORTUS S99

Brucellosis is a worldwide zoonotic disease that caused by Brucella spp. They
are facultative intracellular pathogens which replicate inside macrophages and in non professional
phagocytes cells. The experiences show that medicinal plants can be used successfully to treat
bacterial diseases. The aim of this study is evaluation of bactericidal effects of Eucalyptus globulus
extracts on intramacrophage Brucella abortus S99.
Material & Methods: In this study aquatic, ethanolic and acetonic extracts of Eucalyptus globulus
leaves prepared. Then effect of extracts on intramacrophge surviving of B. abortus S99 studied on cell
culture of Balb/c mice peritoneal macrophages. Therefore, after lysis of macrophages, preparation of
serial dilution and culture on Muller-Hinton Agar media, number of grown CFU counted.
Results: Results indicated that Eucalyptus globulus extracts have antimicrobial activity on
intramacrophage B. abortus S99 after 24h.
Conclusion: Overall cell culture results indicated that Eucalyptus globulus extracts have bactericidal
activity on intramacrophage B. abortus S99 and ethanolic extract has the most effective antimicrobial
activity on intramacrophage brucellae.