Interaction of prenatal stress and morphine alters prolactin and seizure in rat pups

Prenatal exposure to stress andmorphine has complicated effects on epileptic seizure. In the present study, effect
of prenatal forced-swimstress andmorphine co-administration on pentylenetetrazol (PTZ) induced epileptic behaviors
and prolactin blood level (PBL) was investigated in rat offspring. Pregnant Wistar rats were divided to
four groups of control–saline, control–morphine, stressed–saline and stressed–morphine. In the stressed
group, pregnant rats were placed in 25 °C water on gestation days 17, 18 and 19 (GD17, GD18 and GD19) for
30 min. In the morphine/saline group, pregnant rats received morphine (10, 12 and 15 mg/kg, IP, on GD17,
GD18 and GD19, respectively) or saline (1ml, IP). In themorphine/saline-stressed group, the rats received morphine
or saline and then exposed to stress. On postnatal days 6 and 15 (P6 and P15), blood samples were obtained
and PBL was determined. At P15 and P25, the rest of the pups was injected with PTZ to induce seizure.
Then, epileptic behaviors of each rat were observed individually. Latency of first convulsion decreased in control–
morphine and stressed–saline groups while increased in stressed–morphine rats compared to control–saline
group on P15 (P= 0.04). Number of tonic–clonic seizures significantly increased in control–morphine and
stressed–saline rats compared to control–saline group at P15 (P= 0.02). PBL increased in stressed–saline, control–
morphine and stress–morphine groups compared to control–saline rats. It can be concluded that prenatal
exposure of rats to forced-swim stress and morphine changed their susceptibility to PTZ-induced seizure and
PBL during infancy and prepubertal period. Co-administration ofmorphine attenuated effect of stress on epileptic
behaviors.