POLYMORPHISMS OF IFN-Γ AND IL-10 GENES IN NORMAL POPULATION.

Some of polymorphisms are located in the promoter regions or other regulatory sequences of cytokine genes and are associated with increased or decreased amount of cytokines production. The aim of this study was to determine the rate of polymorphisms of IFN-γ +874 and IL-10 -1082 in the normal population.

Materials & Methods: 109 normal individuals were included in the study and genomic description was carried out using ASO-PCR method.

Results: Allelic frequency of alleles A and T for IFN-γ +874 were 50.92% and 49.08% respectively, and the highest genotype frequency was for the genotype A/T (54.13%). Allelic frequency of A and G for IL-10 -1082 also were 50.92% and 49.08% respectively, and genotype A/G had the highest rate among different genotypes of IL-10 -1082 (96.33%).

Conclusion: The alleles A and T of IFN-γ gene (+874) were in Hardy-Weinberg equilibrium, while it was not the case for A and G alleles of IL-10 gene (-1082).