CYTOTOXICITY EFFECT OF KEFIR MICROORGANISMS CYTOPLASMIC EXTRACTS ON GLIOBLASTOMA CANCER CELLS

Kefir is a complex composition of microbial species such as streptococci,
mesophilic lactobacilli, lactose or non-lactose fermenting yeast and acetic acid bacteria. Numerous
studies have been performed to indicate the positive therapeutic effect and healing properties of probiotic
products. The aim of this study was to investigate the effect of cytotoxicity of kefir microorganisms’
cytoplasmic extracts on growth and proliferation of glioblastoma cancer cells as the most acute type of
brain tumors.
Materials & Methods: In this experimental study, kefir microorganisms’ cytoplasmic extract was
prepared. The interaction of U87 glioblastoma cancer cells with different concentrations of cell extracts
and cell wall of kefir microorganisms was studied. Cell proliferation was evaluated using MTT assay
method after 24 and 48 hours.
Results: The results of cytotoxicity test show that the highest effect of cell extracts of kefir
microorganisms on cancer calls was obtained 0.24 and 0.045 mg of extract at 24 and 48 hours,
respectively, and the highest effect of cell wall of kefir microorganisms in both times was a density of
7 mg.
Conclusion: The cell extracts of kefir microorganisms as a probiotic product have a higher toxicity and
fecundity effect on glioblastoma cells. This combination can be suggested as an alternative candidate
for cancer treatment supplement