QUANTITATIVE ASSESSMENT AND MORPHOMETRIC INVESTIGATION OF ANTI-ANGIOGENIC PROPERTIES OF SILVER NANOPARTICLES SYNTHESIZED USING PERSICARIA BISTORTA EXTRACTS ON CHICK CHORIOALLANTOIC MEMBRANE

Angiogenesis is a key event during tumor growth. Since tumor cells require more
oxygen and glucose than normal cells and due to tumors quick growth, new blood vessels rapidly
increase to meet the nutritional requirements. Hence, targeting and inhibiting the formation of new blood
vessels can reduce the nutrition and access of tumor cells to oxygen, which also inhibits tumor growth..
In the present study, the effects of coated silver nanoparticles with Persicaria bistorta on chick embryo
chorioallantoic membrane and its antioxidant properties have been investigated.
Materials & Methods: The fertilized Ross eggs were distributed randomly and equally into 6 groups.
Two groups were considered as control and laboratory control (treated with solvent). In the second day
of incubation, a window was created on the eggs, and then in the 8th day after incubation, samples were
exposed with various doses (60, 120, 240, 480 µg/ml) of the Ag-NPs. On the 12th day, photographs were
taken and the length and number of vessels were measured using Image J software. The length and
weight of embryos were measured and compared. The data were analyzed by SPSS software and one
way ANOVA at the significance level of p <0.05.
Results: Based on the results, the silver nanoparticles at a concentration of 60 μg / ml reduced the length
and number of blood vessels significantly (P<0.001) compared with the control group. The results of
the study on the effect of nanoparticles on the weight of the embryos did not significantly change
compared with the control but, the height of the embryos showed a significant decrease in the treated
groups at a concentration of 240 μg / ml nanoparticles (P<0.01).
Conclusion: Accordingly, the use of silver nanoparticles can be considered as a potential treatment
against pathological angiogenesis and solid tumors targeting blood vessels