ASSESSING THE EFFICIENCY OF PROBIOTIC BACTERIA LACTOBACILLUS ACIDOPHILUS IN DETOXIFICATION OF AFLATOXIN B1 BASED ON A SIMULATED MODEL OF DIGESTIVE SYSTEM SECRETIONS

Since aflatoxin contamination in food and livestock feed is considered as a serious
health problem for human and animal health, this research focused on the ability of Lactobacillus
acidophilus ATCC 4356 in reducing aflatoxin B1 (AFB1) in a simulated human gastrointestinal tract
containing Sterilized milk.
Materials & Methods: For this purpose, the bacteria count and aflatoxin concentration were adjusted to
1×1010 Cfu/ml and 5 ppm, respectively. In the simulated medium, artificial oral salivary, human small
intestine and gastric secretions were inoculated. This study involved 6 treatment in the presence and
absence of bacteria, sterilized milk, and gastrointestinal juice suspension. The concentration of residual
aflatoxin was determined using high-performance liquid chromatography (HPLC) and purification by
Immunoaffinity column. The results were statistically analyzed by SPSS 20 software. The results
indicated that reduction of aflatoxin B1 at all treatments were determined using HPLC with a detection
limit of 0.25mg/ml and a quantification limit of 0.75 mg/ml. The mycotoxin recovery was 89% and 94%
for AFB1 Good linearity was observed for all the analytes of interest, with correlation coefficients 0.995
within their respective linear ranges.
Results: The highest percentage of AFB1 removal by probiotic strain of Lactobacillus acidophilus was
70±0.022% in the absence of sterilized milk, where there was a significant difference for the six groups
(P<0.05).
Conclusion: The results also revealed that probiotic bacteria cell was a good biological agent for
elimination or reduction of AFB1 in the human gastrointestinal tract. In addition to bacteria, gastric juice
and small intestine contribute to elimination or reduction of AFB1