Alteration of delta-6-desaturase (FADS2), secretory phospholipase-A2 (sPLA2) enzymes by Hot-nature diet with co-supplemented hemp seed, evening primrose oils intervention in multiple sclerosis patients

The effect of nutrition and dietary supplements as environmental factors has been suggestedas possible factors affecting both disease risk and progression in on the course of multiple sclerosis withcomplex genetic-risk profiles. This study was aimed to assess regulation of surface-membrane enzymessuch as Delta-6-desaturase (FADS2), secretory Phospholipase A2(sPLA2) by hemp seed and evening prim-rose oils as well as Hot-natured dietary intervention in relapsing remitting multiple sclerosis (RRMS)patients.Methods and materials: In this double blind, randomized trial, 100 RRMS patients with Extended disabilitystatus score (EDSS)<6 were allocated into 3 groups: “Group A” who received co-supplemented hemp seedand evening primrose oils along with advised Hot nature diet; “Group B”, who received olive oil; “GroupC”, who received the co-supplemented oils. Clinically EDSS and functional score as well as biochemicalparameters [blood cells polyunsaturated fatty acid (PUFA), FADS2, sPLA2] were assessed at baseline andafter 6 months.Results: Mean follow-up was 180 ± 2.9SD days (N = 65, 23 M and 42 F aged 34.25 ± 8.07 years with diseaseduration 6.80 ± 4.33 years). There was no significant difference in studies parameters at baseline. After 6months, significant improvements in EDSS and functional score were found in the groups A and C whileEDSS and pyramidal score showed significant increase in group B. Alteration of biochemical parametersshowed improvement in groups A and C whereas there was worsening condition for group B after theintervention.Conclusion: The co-supplemented hemp seed and evening primrose oils with Hot nature diet can havebeneficial effects in improving clinical symptoms and signs in RRMS patients which were confirmed byregulation of surface-membrane enzymes