THE ANTIANGIOGENIC AND CYTOTOXIC PROPERTIES OF GREEN SYNTHESIZED SILVER NANOPARTICLES USING LIQUID EXTRACT OF RAPESEED FLOWER POLLEN

Angiogenesis is an active reaction. In this study, the extract of Rapeseed Flower
Pollen with different antioxidant properties was used to prepare the Bio-green Silver nanoparticle and
then its anti -angiogenic effects of was evaluated. The Bio-green synthesized nanoparticles that were
synthesized by the plant extract have the potential to be used as a reducing agent for metal ions to metal
molecules present in the composition. Today, green nanoparticles are used extensively due to biological
properties such as antioxidant properties, angiogenesis inhibitors, and cancer control.
Materials & Methods: In this test-tube lab research, silver nanoparticles made from rapeseed pollen
were used to investigate anti-angiogenic effects using a CAM assay. For this purpose, 60 eggs (Ross)
were purchased and randomly assigned into 6 groups (n=10): a control group, a laboratory control, and
four treatment groups (25, 50, 100, 200μg/ml). On the second day, the incubation window was made
and on the eighth day, samples were treated with different concentrations of nanoparticles. On the
twelfth day, the photographs were taken from the samples, then the number and the length of the vessels
were evaluated using Imag J software and the height and weight of the embryos were measured. Data
were analyzed by SPSS software and ANOVA test and P <0.05 was considered significant. The MTT
assay was used to evaluate the toxicity of this nanoparticle against liver cancer (HT-29) and (HepG2)
and normal cell (Huvec).
Results: The results of this experiment showed that the nanoparticle are significantly capable of
decreasing the length and the number of blood vessels at concentrations of 100 and 200 μg / ml (p≤0.05).
They are also able to reduce the length and the weight of the treated embryos in comparison to the
control group. The results showed that the silver nanoparticles can significantly inhibit liver cancer cell
(HT-29) and HepG2 (IC50: 0.15 μg/ml) in very low concentrations (IC50:6 μg/ml) but does not have an
inhibitory effect in similar concentration on normal cells (Huvec) (IC50: 0.6 μg/ml) (p ≤ 0.001).
Conclusion: The results showed that the synthesized nanoparticle from rapeseed pollen has an inhibitory
effect on the rate of angiogenesis which makes this nanoparticle a suitable candidate for cancer
treatment. It does not have toxic effects in similar concentrations on Huvec cells, but it is also able to
inhibit the HT-29 and HepG2 cells in a low concentration