ISOLATION OF ESBL GENES FROM PSEUDOMONAS AERUGINOSA ISOLATED FROM CLINICAL SPECIMENS BY MULTIPLEX PCR METHOD

Gram-negative bacterium Pseudomonas aeruginosa (P. aeruginosa) is an
important hospital opportunistic pathogen, due to its intrinsic and acquired resistance to several
antibiotics found in clinical laboratories for identification of Pseudomonas aeruginosa. The aim of this
study was to isolate ESBL beta-lactamase genes from Pseudomonas aeruginosa isolated from clinical
specimens using multiplex PCR.
Materials & Methods: In total, human isolates from different infections that were collected after
isolation of bacteria and extraction of DNA of vim, gim-1, sim-1, imp, spm-1 genes were studied by
multiplex PCR.
Results: Of 70 isolates of human specimens from different infections and of all age groups, sim-1, imp,
vim, -1 spm, and gim-1 genes were used to detect drug resistance in Pseudomonas aeruginosa from the
total clinical samples studied, 61 samples (87.14%) were positive from all strains and VIM, gim-1,and
sim-1 genes were observed in samples. The percentage of vim, gim-1, and sim-1 genes in samples were
21.22, 27.28 and 24.92, respectively. In this regard, imp and spm genes were not observed in the
samples.
Conclusion: Metalobetalactamas is very important due to its resistance to a wide range of antibiotics
used against pseudomonas infections, and the rapid detection of these enzymes in epidemiological and
carbapenem resistant bacteria . In summary, Choosing an effective antibiotic and preventing the spread
of such infections in hospitals is necessary and valuable.