THE EFFECT OF FOUR TRAINING METHODS ON THE COPEPTIN AND MID-REGIONAL PROADRENOMEDULLIN EXPRESSION IN THE LEFT VENTRICLE OF MALE WISTAR RATS

Considering the importance of preventing the risk factors in heart disease and
lack of sufficient information about the effect of exercise training on copeptin and mid-regional
proadrenomedullin expression, this study aimed to investigate the effect of four training methods on the
copeptin and mid-regional proadrenomedullin expression in the left ventricle of male Wistar rats.
Materials & Methods: In this experimental study, 40 male Wistar rats with the age of 8-weeks and an
average weight of 237±33 grams were randomly divided into 5 groups: control, moderate-intensity
training, high-intensity training, high-intensity interval training, and moderate-intensity swimming
training. The training programs in experimental groups were performed for 8 weeks (5 days a week).
Expression of research genes was determined by real-time polymerase chain reaction. To analyze the
data, one way ANOVA and bonferroni were used at a significance level of p≤ 0.05.
Results: Copeptin and mid-regional proadrenomedullin expressions in each training group decreased
significantly compared to the control group (p= 0.001, p= 0.001). Also, Copeptin and mid-regional
proadrenomedullin expression increased significantly in HIIT and HIT groups compared to ST and MIT
groups (p= 0.001, p= 0.001).
Conclusion: All of the four exercise training methods were able to reduce the expression of the studied
genes, and make favorable changes in reducing the consequences of cardiac tissue injury. However,
swimming exercises seem to have more favorable effects with regard to non-weight bearing property,
although further research is needed