Topical application of Dianthus essential oil improved the infected healing process of wounds infected with Staphylococcus aureus in an experimental model

In recent years, wound infections caused by Staphylococcus aureus have substantially grown. Lack of easy
access, medications expensive, side effects, and in particular the development of drug resistance, the use of biological materials are
proposed as an alternative solution. The recent study was aimed to evaluate the topical effect of Dianthus essential oil on cutaneous
excisional wound healing in mice infected with S. aureus.
Materials & Maethods: This study was performed on 36 mice (weight 25±3 g). After general anesthesia, 0.5 mm circle wound was
created with biopsy punch between the shoulder, and immediately 50 γ of the suspension containing 107 CFU/ml S. Aureus was applied
to the wound. Then tested animals, grouping in three groups of 12 mice each (control, treated with ointment 2% and 4%). During the
project, image was obtained on days 3, 6, 9 and 12 images for assessment of wound area, and in 3, 7 and 14 from wounds in order to
histopathology assessment.
Results: The results of the wound size showed that the wound area decreased significantly in the treated groups (p< 0.05) compared
with the control group. On histological examination, a significant (p< 0.05) reduction was observed in the migration of immune cells,
the migration of fibroblasts and fibrocytes into the wound, the collagen synthesis and secretion. In addition, the thickness of the
epithelium thickest increased in the treatment groups compared with the control group (p< 0.05).
Conclusion: Based on the results of this study, topical application of Dianthus essential oil, especially at higher therapeutic doses, can
be considered a viable option for treatment of infected wounds by the bacteria S. aureus