Anti-inflammatory Effects of Memantine in Carrageenan-induced Paw Edema Model in Rats

The anti-inflammatory effect of memantine (MEM) was investigated using carrageenaninduced
hind paw edema model in rats. Materials and Methods: Thirty male Wistar rats were randomly
assigned to five groups (n = 6). Group 1 received 0.1 mL of 1% carrageenan at the right hind paw.
Group 2 received dexamethasone (10 mg/kg) and Groups 3, 4, and 5 received 5, 10, and 20 mg/kg MEM
intraperitoneally (ip), 20 min after injection of carrageenan into the right hind paw, respectively. Animals’
paw thickness was measured at 0, 1, 2, 3, 4, and 5 h after carrageenan injection. Then, animals were
euthanized and myeloperoxidase (MPO) and malondialdehyde (MDA) levels were measured in the paw
tissues. The tissue samples were further examined histopathologically using light microscopy. One-way
ANOVA and Tukey post hoc test was used to compare the mean values between the groups. Results:
Treating with MEM at all doses significantly decreased hind paw thickness at 2 (P < 0.05 and P < 0.01 at
MEM 10 mg/kg and MEM 5 and 20 mg/kg, respectively), 3 (P < 0.001), and 4 (P < 0.001 at 5 mg/kg and
P < 0.01 at MEM 10 and 20 mg/kg) hours after carrageenan injection in comparison to the carrageenan
group. There was a significant (P < 0.05 and P < 0.001, respectively) reduction in MPO activity and
MDA levels in MEM-treated groups when compared with the carrageenan group. Conclusion: This
study showed that MEM decreased paw edema, leukocyte infiltration, MPO activity, and MDA levels,
and MEM can be considered as an effective anti-inflammatory agent