SIRTUIN 6 GENE EXPRESSION AND TELOMERE LENGTH CHANGES IN OLD RAT HEPATOCYTES AFTER 6 WEEKS SWIMMING EXERCISE AND RESVERATROL SUPPLEMENTATION FEEDING

telomere erosion is considered to be the main cause of aging. The present study
aimed to determine the interactive effect of high-intense interval training and resveratrol consumption
on telomere length and expression sirtuin 6 genes of hepatocytes in elderly rats.
Materials & Methods: 25 Wistar male rats were divided into 5 groups. 1. Control group that did not
exercise, 2. The solvent group that only received the solvent, 3. Exercise actively group that swam 20
seconds with 10 seconds rest in 6 weeks and 3 sessions per week, 4. Supplement group that received
resveratrol 10 ml/kg per day, and 5. Combination of exercise and supplement goup that uses combination
of exercise and supplement. Quantitative Real time PCR was used to measure the expression levels of
sirtuin 6 gene and telomere length. For data analysis, One-way analysis of variance, and tukey tests were
done usingSPSS 25 software.
Results: Resveratrol consumption and exercise significantly increased telomere length compared to
control in order (P=0.000, 0.001) and solvent (p=0.007, 0.004) groups, respectively. The expression of
the sirtuin 6 gene was not affected by resveratrol (0.984), while exercise increased the expression of the
sirtuin 6 gene (0.013). The combination of resveratrol supplementation and exercise did not double
telomere length (0.395) and sirtuin 6 expression (0.502).
Conclusion: The interaction of very high-intensity interval training and resveratrol supplementation
does not double the expression of the sirtuin 6 gene and telomere length of old rat liver cells. The
molecular mechanism for slowing telomere erosion is probably complex and unaffected by mentioned
concurrent interventions.