Identification of echinococcus granulosus strains in isolated hydatid cyst specimens from animals by PCR-RFLP method in West Azerbaijan - Iran

The aim of this study was DNA extraction from protoscolecses
of Echinococcus granulosus and identification of these strains in West-
Azerbaijan Province, north western Iran.
Methods: Thirty one livestock isolates from sheep and cattle were collected
from abattoirs of the province. To investigate the genetic variation of the
isolates, after DNA extraction by Glass beads-phenol chloroform method;
PCR-RLFP analysis of rDNA-ITS1 was performed using three different restriction
enzymes of Taq 1, Rsa 1 and Alu 1.
Result: Amplified PCR products for all isolates were 1000bp band which is
expected band in sheep strains (G1-G3 complex). The results of RFLP analysis
also were the same for all isolates. PCR-RFLP patterns restriction enzymes
were identical as follows, Rsa1 bands under UV showed two bands
approximately 655bp and 345bp. Alu1 bands were as follows: two approximately
800bp and 200bp and Taq1 did not cut any region and bands were
approximately 1000 bp in all samples.
Conclusions: Based on PCR-RFLP patterns of ITS1 fragment produced
with endonucleases enzyme digestion in animal isolates, it can be concluded
that a single strain of E. granulosus (sheep strain or G1-G3 complex) is dominant
genotype in this province