HEALING EFFECTS OF ION-EXCHANGED ZEOLITE NANOCOMPOSITE ON THIRD -DEGREE BURN WOUND IN MICE

Zeolite has skin wound healing capacity. However, its efficacy on burn wound
healing has not yet been examined. In this study, we investigated the effects of Zeolite-Ag+/Gelatin
nanocomposite on the process of third-degree burn wound healing in mice model.
Materials & Methods: Ag+- zeolite/gelatin nanocomposite was fabricated by sol-gel method; MTT
assay and antimicrobial activity evaluation of the nanocomposite were performed. Third degree burn
wound were created on 40 Balb/c mice (n=10) wound per group and treatment administered daily
starting on the day of wounding. Treatment groups included untreated control, SSD, gelatin and
nanocomposite. Daily photographs were taken and change in wound area relative to initial area was
calculated. At 7 and 14 days, animals euthanized and skin samples were taken to histopathologicl
evaluation (H&E staining). Then, the study groups were evaluated for wound contraction percent and
histopathological parameters.
Results: According to the results of the present study, nano composite-treated wounds revealed
significant wound contraction on days 14 compared to control and gelatin treated groups (P<0.0001).
Considering investigated histopathological parameters including re-epithelialization angiogenesis and
fibroplasia in mice treated with nanocomposite the rate healing of was significantly accelerated when
compared to the other groups.
Conclusion: In conclusion, based on the results of the present study, it could be concluded that zeoliteAg/gelatin nanocomposite offered potential advantages in burn wound healing acceleration and
improvement. However, clinical use of this nanocomposite needs more supportive studies.