Isolation, Molecular Identification and Characterization of the Culture Conditions for Extracellular Uricase Production by a New Strain of Pseudomonas sp

Ghasemian, A and Baniasad, M and Moradpour, Z and Ghasemi, Y (2015) Isolation, Molecular Identification and Characterization of the Culture Conditions for Extracellular Uricase Production by a New Strain of Pseudomonas sp. JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 9 (4). pp. 2813-2823.

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Abstract

An uricase producing bacteria was isolated from soil through a screening program
with a medium containing uric acid as sole carbon source. On the basis of the biochemical
and phenotypic data, together with molecular analysis based on 16S rDNA gene sequences,
this strain identified as a novel species of the genus Pseudomonas. The optimum conditions
for uricase production by this Pseudomonas isolate were determined by studying the
effect of some environmental and cultural factors. Among seven variables tested in our
study to optimize uricase production by Pseudomonas sp., carbon and nitrogen sources,
pH and CuSO4 were selected based on their high significant effect on uricase production.
The optimal combination of the major constituents of media for uricase production
evaluated from the optimization algorithm of Box-Behnken was as follows: glucose,
0.1%; pH, 7; yeast extract, 0.5%; and CuSO4, 0.001g

Item Type: Article
Uncontrolled Keywords: Uricase; Pseudomonas; Optimization; Isolation
Subjects: R Medicine > R Medicine (General)
Depositing User: Unnamed user with email gholipour.s@umsu.ac.ir
Date Deposited: 02 Oct 2018 06:11
Last Modified: 27 Jan 2019 08:38
URI: https://eprints.umsu.ac.ir/id/eprint/5138

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